For more information: http://www.7activestudio.com http://www.7activemedical.com/ 7activestudio@gmail.com Contact: +91- 9700061777, 040-64501777 / 65864777 7 Active Technology Solutions Pvt.Ltd. is an educational 3D digital content provider for K-12. We also customise the content as per your requirement for companies platform providers colleges etc . 7 Active driving force "The Joy of Happy Learning" -- is what makes difference from other digital content providers. We consider Student needs, Lecturer needs and College needs in designing the 3D & 2D Animated Video Lectures. We are carrying a huge 3D Digital Library ready to use. GENETIC ENGINEERING: In vitro DNA technology used to isolate genes from an organism, manipulate them in laboratory as per desire and insert them into another cell system for specific genetic trait. The conceptual development of the principle of genetic engineering: The techniques of genetic engineering include creation of recombinant DNA, use of gene cloning and gene transfer which helps to isolate and introduce only one or a set of desirable genes without introducing undesirable genes into the target organism.When a piece of DNA is introduced into alien organism, this piece of DNA would not be able to multiply itself in the progeny cells of the organism. But, when it gets integrated into the genome of the recipient, it may multiply and be inherited along with the host DNA. This is because; the alien piece of DNA has become the part of the chromosome, which has the ability to replicate.The construction of an artificial recombinant DNA molecule emerged from the possibility of linking a gene encoding antibiotic resistance with the native plasmid of Salmonella typhimurium. Stanley Cohen & Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance. The cutting of DNA at specific locations became possible with the discovery of 'molecular scissors' - restriction enzymes. The cut piece of DNA was then linked with plasmid DNA. These plasmid DNA act as vectors to transfer the piece of DNA attached to it. Plasmid is used as vector to deliver an alien piece of DNA into the host organism. The linking of antibiotic resistance gene with the plasmid vector became possible with enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replicating DNA created in vitro and is known as recombinant DNA.When DNA is transferred into E. coli, it could replicate using the new hosts DNA polymerase enzyme and make multiple copies. The ability to multiply copies of antibiotic resistance gene in E. coli was called cloning of antibiotic resistance gene in E. coli.

Biotech 101 is a four-part lecture series hosted by the HudsonAlpha Institute for Biotechnology, a 501(c)(3) non-profit organization in Huntsville, Alabama. The 2021 series is sponsored by Twickenham Advisors - a Hightower company. Session 1 Handouts - https://bit.ly/3DPnMqJ Join the HudsonAlpha Alumni Association - https://bit.ly/3E5NOpQ

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